Recombinant Human Lysosome-Associated Membrane Glycoprotein 1 (LAMP1) Protein (hFc), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: P11279
• Gene: LAMP1
• CAT.NO: B000270
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human Lysosome-Associated Membrane Glycoprotein 1 (LAMP1) Protein (hFc), Active
• Endotoxin: Less than 1.0 EU/μg as determined by LAL method.
• Protein Expression Range: 29-382aa
• Tag: C-hFc
• Molecular Mass: 65.5 kDa
• Form: Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.; 2. Avoid repeated freeze-thaw cycles.; 3. Store working aliquots at 4°C for up to one week.; 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: The ED50 as determined by its ability to bind Human Galectin-3 in functional ELISA is less than 20 ng/ml.
• Research Area: Cancer
• Protein Length: Partial

This lysosomal membrane glycoprotein plays a crucial role in lysosome biogenesis, pH regulation, autophagy, and cholesterol homeostasis. It regulates lysosomal lumen pH by directly inhibiting the proton channel TMEM175, aiding in lysosomal acidification for optimal hydrolase activity. The protein also plays an essential role in natural killer (NK) cell cytotoxicity. It participates in the movement of cytotoxic granules to the cell surface and the trafficking of perforin to the lytic granules. Additionally, it protects NK cells from degranulation-induced damage caused by their own cytotoxic granule contents and presents carbohydrate ligands to selectins.