Recombinant Human Melanocyte-stimulating hormone receptor (MC1R)-VLPs

  • Purity: Greater than 95% as determined by SEC-HPLC.
  • Host Species: Homo sapiens (Human)
  • Accession: Q01726
  • Gene: MC1R
  • CAT.NO: B000113
  • Expression Systems:

    Mammalian cell

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Product Name Recombinant Human Melanocyte-stimulating hormone receptor (MC1R)-VLPs
Accession Q01726
Purity Greater than 95% as determined by SEC-HPLC.
Host Species Homo sapiens (Human)
Gene MC1R
Source Mammalian cell
Protein Expression Range 1-317aa
Tag C-terminal 10xHis-tagged(This tag can be tested only under denaturing conditions)
Molecular Mass 36.1 kDa
Form Liquid or Lyophilized powder
Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Research Area Signal Transduction
Alternative Names MSH-R;Melanocortin receptor 1;MC1-R
Target Protein Sequence MAVQGSQRRLLGSLNSTPTAIPQLGLAANQTGARCLEVSISDGLFLSLGLVSLVENALVVATIAKNRNLHSPMYCFICCLALSDLLVSGSNVLETAVILLLEAGALVARAAVLQQLDNVIDVITCSSMLSSLCFLGAIAVDRYISIFYALRYHSIVTLPRARRAVAAIWVASVVFSTLFIAYYDHVAVLLCLVVFFLAMLVLMAVLYVHMLARACQHAQGIARLHKRQRPVHQGFGLKGAVTLTILLGIFFLCWGPFFLHLTLIVLCPEHPTCGCIFKNFNLFLALIICNAIIDPLIYAFHSQELRRTLKEVLTCSW
Protein Length Full Length
Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).

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