Recombinant Human Microtubule-Associated Protein Tau (MAPT) Protein (His), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Human
• Accession: P10636
• Gene: MAPT
• CAT.NO: B000069
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human Microtubule-Associated Protein Tau (MAPT) Protein (His), Active
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Protein Expression Range: 1-441aa
• Tag: N-10His
• Molecular Mass: 49.5 kDa
• Form: Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
• Storage: 1. Store at -20～C/-80～C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4～C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20～C/-80～C. Protein in lyophilized powder form is stable for up to 12 months at -20～C/-80～C.
• Bioactivity: Measured by its binding ability in a functional ELISA. Immobilized Human MAPT at 2 μg/ml can bind Anti-MAPT recombinant antibody , the EC 50 is 4.547-6.284 ng/mL.

Tau protein promotes microtubule assembly and stability and plays a critical role in establishing and maintaining neuronal polarity. Its C-terminus binds to axonal microtubules, while its N-terminus interacts with neural plasma membrane components, functioning as a linker between the cytoskeleton and membrane structures. Axonal polarity is determined by the localization of Tau within the neuronal cell body, specifically in the domain defined by the centrosome.
Tau isoforms contribute differently to cytoskeletal dynamics. Short isoforms enhance cytoskeletal plasticity, allowing dynamic remodeling, whereas longer isoforms are primarily involved in stabilizing the microtubule network. This functional versatility underscores Tau’s importance in neuronal development, polarity, and maintenance, as well as its potential implications in disorders involving cytoskeletal dysfunction.