Recombinant Human Muscarinic Acetylcholine Receptor M1 (CHRM1) Protein (His)

• Purity: Greater than 85% as determined by SDS-PAGE.
• Host Species: Human
• Accession: P11229
• Gene: CHRM1
• CAT.NO: B000068
• Expression Systems: E.coli
• Product Name: Recombinant Human Muscarinic Acetylcholine Receptor M1 (CHRM1) Protein (His)
• Protein Expression Range: 210-366aa
• Tag: N-10His
• Molecular Mass: 20.8 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

The muscarinic acetylcholine receptor regulates diverse cellular responses by interacting with G proteins. It inhibits adenylate cyclase, promotes phosphoinositide breakdown, and modulates potassium channels, influencing intracellular signaling pathways. Its primary transducing mechanism involves phosphoinositide (Pi) turnover, generating second messengers crucial for cellular responses. This receptor plays a key role in physiological processes such as neurotransmission, smooth muscle contraction, and glandular secretion. By coupling extracellular acetylcholine signals to intracellular pathways, it helps maintain cellular and systemic homeostasis, highlighting its importance in both neural and non-neural functions.