Recombinant Human Muscarinic Acetylcholine Receptor M2 (CHRM2) Protein (His)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Human
• Accession: P08172
• Gene: CHRM2
• CAT.NO: B000082
• Expression Systems: E.coli
• Product Name: Recombinant Human Muscarinic Acetylcholine Receptor M2 (CHRM2) Protein (His)
• Protein Expression Range: 210-387aa
• Tag: N-6His
• Molecular Mass: 23.4 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

The muscarinic acetylcholine receptor mediates diverse cellular responses by activating G proteins. Its primary transducing effect is the inhibition of adenylate cyclase, reducing cyclic AMP levels. Additionally, receptor signaling activates phospholipase C, leading to the hydrolysis of phosphoinositides and the generation of inositol trisphosphate (IP3). IP3 subsequently triggers the release of calcium ions from intracellular stores into the cytosol, facilitating various downstream signaling processes. The receptor also modulates potassium channels, influencing membrane potential and cellular excitability. Through these mechanisms, the muscarinic acetylcholine receptor regulates vital physiological functions such as neurotransmission, smooth muscle contraction, and glandular secretion, highlighting its importance in maintaining cellular signaling and systemic homeostasis.