Recombinant Human Muscarinic Acetylcholine Receptor M3 (CHRM3) Protein (His)

• Purity: Greater than 85% as determined by SDS-PAGE.
• Host Species: Human
• Accession: P20309
• Gene: CHRM3
• CAT.NO: B000057
• Expression Systems: E.coli
• Product Name: Recombinant Human Muscarinic Acetylcholine Receptor M3 (CHRM3) Protein (His)
• Protein Expression Range: 253-492aa
• Tag: N-10His
• Molecular Mass: 32.7 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

The muscarinic acetylcholine receptor mediates diverse cellular responses by activating G proteins, influencing key signaling pathways. It inhibits adenylate cyclase, promotes the breakdown of phosphoinositides, and modulates potassium channel activity, contributing to a wide range of physiological effects. Its primary transducing mechanism involves phosphoinositide (Pi) turnover, which generates second messengers critical for intracellular signaling. Through these actions, the receptor plays a pivotal role in regulating processes such as neurotransmission, smooth muscle contraction, and glandular secretion, highlighting its importance in maintaining cellular and systemic homeostasis.