Recombinant Human Muscarinic Acetylcholine Receptor M5 (CHRM5) Protein (His)

• Purity: Greater than 85% as determined by SDS-PAGE.
• Host Species: Human
• Accession: P08912
• Gene: CHRM5
• CAT.NO: B000076
• Expression Systems: E.coli
• Product Name: Recombinant Human Muscarinic Acetylcholine Receptor M5 (CHRM5) Protein (His)
• Protein Expression Range: 215-443aa
• Tag: N-6His
• Molecular Mass: 31.5 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

The muscarinic acetylcholine receptor mediates diverse cellular responses by interacting with G proteins. It inhibits adenylate cyclase, promotes phosphoinositide breakdown, and modulates potassium channels, playing a key role in intracellular signaling. Its primary transducing mechanism involves phosphoinositide (Pi) turnover, generating second messengers essential for cellular processes. This receptor regulates physiological functions such as neurotransmission, smooth muscle contraction, and glandular secretion, highlighting its importance in maintaining cellular and systemic homeostasis.