Recombinant Human Natural resistance-associated macrophage protein 2 (SLC11A2), partial

• Purity: >85% (SDS-PAGE)
• Host Species: Homo sapiens (Human)
• Accession: P49281
• Gene: SLC11A2
• CAT.NO: B000288
• Expression Systems: E.coli In Vivo Biotinylation in E.coli Yeast Baculovirus Mammalian cell
• Product Name: Recombinant Human Natural resistance-associated macrophage protein 2 (SLC11A2), partial
• Source: E.coli;In Vivo Biotinylation in E.coli;Yeast;Baculovirus;Mammalian cell
• Tag: Tag type will be determined during the manufacturing process.
• Form: Lyophilized powder
• Buffer: Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Alternative Names: DCT 1; dct-1; DCT1; Divalent cation transporter 1; Divalent metal transporter 1; DMT 1; DMT-1; DMT1; FLJ37416; Natural resistance associated macrophage protein 2; Natural resistance-associated macrophage protein 2; NRAM2_HUMAN; NRAMP 2; NRAMP2; OK/SW-cl.20; Slc11a2; Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2; Solute carrier family 11 member 2
• Protein Length: Partial
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

This proton-coupled metal ion symporter operates with a 1:1 stoichiometry of protons to metal ions. It selectively transports various divalent metal cations in the following order of affinity: Cd2+ > Fe2+ > Co2+, Mn2+ >> Zn2+, Ni2+, VO2+. It is crucial for maintaining iron homeostasis by regulating the intestinal absorption of dietary Fe2+ and the transport of transferrin-associated endosomal Fe2+ in erythroid precursors and other cells. The transporter facilitates the entry of Fe2+ and Mn2+ ions into mitochondria, supporting mitochondrial heme synthesis, iron-sulfur cluster biogenesis, and antioxidant defense. It can also mediate uncoupled fluxes of protons or metal ions.