Recombinant Human Non-Receptor Tyrosine-Protein Kinase Tyk2 (TYK2) Protein (His)

• Purity: Greater than 85% as determined by SDS-PAGE.
• Host Species: Human
• Accession: P29597
• Gene: TYK2
• CAT.NO: B000044
• Expression Systems: E.coli
• Product Name: Recombinant Human Non-Receptor Tyrosine-Protein Kinase Tyk2 (TYK2) Protein (His)
• Protein Expression Range: 556-871aa
• Tag: N-6His
• Molecular Mass: 39.3 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

TYK2 is a non-receptor tyrosine kinase critical for signaling by various cytokines and interferons, regulating cell growth, development, migration, and immune responses. It plays both structural and catalytic roles in signaling pathways mediated by interleukins and type I interferons (IFN-alpha/beta). TYK2 associates with heterodimeric cytokine receptor complexes, which include a TYK2-associated receptor chain (e.g., IFNAR1, IL12RB1, IL10RB, or IL13RA1) and a second receptor chain paired with JAK1 or JAK2.
Upon cytokine binding, TYK2 phosphorylates and activates its associated receptor chains, creating docking sites for STAT family members, such as STAT1, STAT3, STAT4, and STAT6. These STATs are phosphorylated by TYK2 (or JAK1/JAK2 on the second chain), forming homo- or heterodimers that translocate to the nucleus to regulate gene expression. TYK2 also negatively regulates STAT3 activity by promoting phosphorylation at a distinct tyrosine site, modulating signaling to ensure proper cellular responses.