Recombinant Human Nuclear Receptor Subfamily 1 Group I Member 2 (NR1I2) Protein (His-SUMO&Myc)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Human
• Accession: O75469
• Gene: NR1I2
• CAT.NO: B000106
• Expression Systems: E.coli
• Product Name: Recombinant Human Nuclear Receptor Subfamily 1 Group I Member 2 (NR1I2) Protein (His-SUMO&Myc)
• Protein Expression Range: 1-434aa
• Tag: N-10His-SUMO&C-Myc
• Molecular Mass: 69.8kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

This nuclear receptor is a transcription factor activated by a wide range of endogenous and xenobiotic compounds. It plays a key role in regulating the expression of genes involved in the metabolism and clearance of potentially harmful substances, including drugs, toxins, and endogenous metabolites. The receptor is activated by various ligands, such as the antibiotic rifampicin, plant-derived compounds like hyperforin, guggulipid, colupulone, and isoflavones, as well as naturally occurring steroids like pregnenolone and progesterone.
Upon activation, it binds to specific response elements in gene promoters, such as those of CYP3A4 and ABCB1/MDR1, driving their transcription. These genes encode proteins critical for drug metabolism and transport, including cytochrome P450 enzymes and multidrug resistance transporters. Notably, the receptor’s response to ligands is species-specific, emphasizing its role in adapting to diverse environmental and physiological challenges.