Recombinant Human Peroxisome Proliferator-Activated Receptor Delta (PPARD) Protein (His)

• Purity: Greater than 85% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: Q03181
• Gene: PPARD
• CAT.NO: B000218
• Expression Systems: E.coli
• Product Name: Recombinant Human Peroxisome Proliferator-Activated Receptor Delta (PPARD) Protein (His)
• Protein Expression Range: 171-441aa
• Tag: N-6His
• Molecular Mass: 35.1 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

This ligand-activated transcription factor is a key mediator of energy metabolism in adipose tissue. It binds peroxisome proliferators, including hypolipidemic drugs and fatty acids, with a preference for polyunsaturated fatty acids like gamma-linoleic acid and eicosapentaenoic acid. Upon ligand activation, the receptor binds to promoter elements of target genes, regulating the peroxisomal beta-oxidation pathway of fatty acids. It functions as a transcriptional activator for the acyl-CoA oxidase gene and, once activated, decreases the expression of NPC1L1, contributing to lipid metabolism regulation.