Recombinant Human Phosphatidylinositol 4,5-Bisphosphate 3-Kinase Catalytic Subunit Gamma Isoform (PIK3CG) Protein (His&Myc)

• Purity: Greater than 85% as determined by SDS-PAGE.
• Host Species: Human
• Accession: P48736
• Gene: PIK3CG
• CAT.NO: B000028
• Product Name: Recombinant Human Phosphatidylinositol 4,5-Bisphosphate 3-Kinase Catalytic Subunit Gamma Isoform (PIK3CG) Protein (His&Myc)
• Source: E.coli
• Protein Expression Range: 828-1073aa
• Tag: N-10His&C-Myc
• Molecular Mass: 35.4 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

Phosphoinositide-3-kinase (PI3K) gamma catalyzes the phosphorylation of PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to produce PIP3 (phosphatidylinositol 3,4,5-trisphosphate), a critical signaling molecule that recruits PH domain-containing proteins such as AKT1 and PDPK1 to the membrane. This process activates downstream signaling pathways involved in cell growth, survival, proliferation, motility, and morphology. PI3K gamma links G-protein-coupled receptor (GPCR) activation to PIP3 production and plays vital roles in immune, inflammatory, and allergic responses.
PI3K gamma regulates leukocyte chemotaxis, polarization, and migration by orchestrating PIP3 spatial distribution, F-actin organization, and integrin-based adhesion at the leading edge. It controls dendritic cell motility and, along with PIK3CD, is essential for natural killer (NK) cell development and migration, T-lymphocyte migration, proliferation, activation, cytokine production, and development. It also contributes to B-lymphocyte signaling and neutrophil respiratory bursts, chemotaxis, and extravasation. Additionally, it cooperates with PIK3CB in platelet aggregation and thrombosis, modulating alpha-IIb/beta-3 integrins’ adhesive functions in platelets.
In cardiac tissue, PI3K gamma negatively regulates contractility by anchoring PKA and PDE3B, lowering cAMP levels. It also promotes beta-adrenergic receptor internalization via interactions with GRK2 and non-muscle tropomyosin phosphorylation, contributing to beta-adrenergic receptor endocytosis. Its lipid and serine/threonine kinase activities also play roles in pathological cardiac hypertrophy. Furthermore, PI3K gamma participates in angiogenesis via a signaling complex involving RAPGEF3 and PDE3B.
In neutrophils, PI3K gamma functions downstream of RASGRP4-mediated Ras activation in GPCR signaling, promoting phospholipase C activation and neutrophil functional responses. This multifunctional kinase integrates immune, vascular, and cardiac signaling pathways.