Pyruvate kinase is an essential enzyme in glycolysis that catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, coupled with the production of ATP. This reaction represents the final step of glycolysis and is crucial for cellular energy production. By facilitating this key metabolic process, pyruvate kinase supports energy generation and provides pyruvate, a critical substrate for the tricarboxylic acid (TCA) cycle and other metabolic pathways. Its activity is tightly regulated to ensure proper energy balance and metabolic flux within the cell, highlighting its central role in maintaining cellular function and energy homeostasis.
| Product Name | Recombinant Human PKLR Protein (C-6His) |
| Accession | P30613 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Host Species | Human |
| Gene | PKLR |
| Source | Human Cells |
| Protein Expression Range | Met1-Ser574 |
| Tag | C-6His |
| Molecular Mass | 62.9 KDa |
| Buffer | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 5% Trehalose, 5% Mannitol, 0.02% Tween 80, 50% Glycerol, 1mM EDTA, 1mM DTT, pH8.0. |
| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
