The beta subunit of the gastric H+/K+ ATPase pump facilitates the exchange of H+ and K+ ions across the apical membrane of parietal cells, crucial for gastric acid secretion. It plays a structural and regulatory role, ensuring proper assembly and membrane targeting of the active pump (by similarity).
During the transport cycle, ATP hydrolysis drives the transfer of H+ ions, involving transient phosphorylation of the alpha subunit. This phosphorylation shifts the pump from an inward-facing (E1) to an outward-facing (E2) conformation. The beta subunit interacts with the alpha subunit’s phosphorylation domain, acting as a stabilizing ratchet that locks the pump in the lumenal-open E2 state, preventing the reverse reaction and ensuring unidirectional ion transport (by similarity). Its role is essential for the proper function of the gastric H+/K+ ATPase and effective acid regulation.
| Product Name | Recombinant Human Potassium-Transporting Atpase Subunit Beta (ATP4B) |
| Accession | P51164 |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Host Species | Human |
| Gene | ATP4B |
| Source | E.coli |
| Protein Expression Range | 58-291aa |
| Tag | Tag-Free |
| Molecular Mass | 26.6kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
