Recombinant Human Potassium Voltage-Gated Channel Subfamily A Member 1 (KCNA1) Protein (His)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Human
• Accession: Q09470
• Gene: KCNA1
• CAT.NO: B000016
• Expression Systems: E.coli
• Product Name: Recombinant Human Potassium Voltage-Gated Channel Subfamily A Member 1 (KCNA1) Protein (His)
• Protein Expression Range: 1-154aa
• Tag: N-6His
• Molecular Mass: 22.2kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

The voltage-gated potassium channel KCNA1 mediates transmembrane potassium transport in excitable membranes, predominantly in the brain and central nervous system, but also in the kidney. It regulates membrane potential and nerve signaling, preventing neuronal hyperexcitability. KCNA1 forms tetrameric potassium-selective channels, which open and close in response to voltage changes across the membrane, facilitating potassium ion flux along its electrochemical gradient.
KCNA1 can assemble into functional homotetrameric or heterotetrameric channels with other family members like KCNA2, KCNA4, and KCNA5. The channel’s properties depend on the alpha subunit composition, while cytoplasmic beta subunits modulate subcellular localization and promote rapid inactivation. Homotetrameric KCNA1 channels act as delayed rectifiers, opening with membrane depolarization and closing slowly. In contrast, heterotetrameric channels, such as KCNA1/KCNA4, inactivate rapidly.
KCNA1 regulates neuronal excitability in the hippocampus, vagus nerve, and cerebellum, contributing to pacemaking, action potential generation, and neurotransmitter release, including GABA and dopamine. It influences pain perception from mechanical stimuli but not heat, aids in auditory localization, and supports postnatal brain development and neuronal precursor proliferation. In the kidney, KCNA1 regulates magnesium ion reabsorption in the distal convoluted tubules, contributing to magnesium homeostasis. KCNA1 is essential for normal neuromuscular responses and heart contraction regulation.