Recombinant Human Rac-Alpha Serine/Threonine-Protein Kinase (AKT1) Protein (GST)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Human
• Accession: P31749
• Gene: AKT1
• CAT.NO: B000040
• Product Name: Recombinant Human Rac-Alpha Serine/Threonine-Protein Kinase (AKT1) Protein (GST)
• Source: E.coli
• Protein Expression Range: 1-480aa
• Tag: N-GST
• Molecular Mass: 82.7kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C.

AKT1, a serine/threonine kinase, is one of three closely related AKT isoforms (AKT1, AKT2, AKT3) that regulate key processes, including metabolism, proliferation, survival, growth, and angiogenesis. It phosphorylates over 100 downstream substrates, though isoform-specific functions remain unclear. AKT1 mediates insulin-induced glucose uptake by promoting the translocation of SLC2A4/GLUT4 to the cell surface. It also inhibits phosphatase activity of PTPN1 by phosphorylating ‘Ser-50,’ enhancing insulin receptor signaling.
AKT1 phosphorylates TBC1D4, enabling its interaction with 14-3-3 proteins, which is critical for insulin-stimulated glucose transport. It regulates glycogen storage by phosphorylating GSK3A and GSK3B, inhibiting their kinase activity. Cell survival is promoted by phosphorylating MAP3K5 at ‘Ser-83,’ suppressing apoptosis under oxidative stress. AKT1 also activates the mTORC1 pathway via phosphorylation of TSC2, regulating protein synthesis and cellular growth.
AKT1 modulates FOXO transcription factors (e.g., FOXO1, FOXO3, FOXO4) through phosphorylation, promoting cytoplasmic localization and inhibiting pro-apoptotic functions. It regulates NF-kappa-B-dependent transcription and activates CREB1, driving pro-survival gene expression. Additionally, AKT1 phosphorylates substrates like ACLY (‘Ser-454’) to influence fatty acid synthesis and PIKFYVE (‘Ser-318’) to enhance PI3P-5 activity.
AKT1 plays a pivotal role in adult neurogenesis, placental development, cell migration, and adhesion. It phosphorylates multiple kinases, including STK4, STK3, and RAF1, influencing diverse cellular processes, including apoptosis, signaling, and growth.