Recombinant Human Raf Proto-Oncogene Serine/Threonine-Protein Kinase (RAF1) Protein (His-SUMO)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: P04049
• Gene: RAF1
• CAT.NO: B000216
• Expression Systems: E.coli
• Product Name: Recombinant Human Raf Proto-Oncogene Serine/Threonine-Protein Kinase (RAF1) Protein (His-SUMO)
• Protein Expression Range: 1-648aa
• Tag: N-6His-SUMO
• Molecular Mass: 89.1kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

RAF1 is a serine/threonine kinase that acts as a key regulatory link between Ras GTPases and the MAPK/ERK cascade, influencing cell fate decisions such as proliferation, differentiation, apoptosis, survival, and oncogenic transformation. It activates a MAPK cascade involving MAP2K1/MEK1, MAP2K2/MEK2, MAPK3/ERK1, and MAPK1/ERK2. RAF1 phosphorylates BAD/Bcl2-antagonist of cell death, adenylyl cyclases, PPP1R12A, and TNNT2. It regulates NF-kB activation, Rho signaling, and migration, and plays a role in wound healing and oncogenic transformation by modulating occludin and SNAI2/SLUG. RAF1 also protects against apoptosis and restricts caspase activation.