Recombinant Human SARS coronavirus Replicase polyprotein 1ab (rep), partial,Yeast

• Purity: >85% (SDS-PAGE)
• Host Species: Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
• Accession: P0C6X7
• Gene: rep
• CAT.NO: B000299
• Expression Systems: Yeast
• Product Name: Recombinant Human SARS coronavirus Replicase polyprotein 1ab (rep), partial,Yeast
• Tag: N-terminal His-taggedTag-Free
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Alternative Names: rep; 1a-1b; Replicase polyprotein 1ab; pp1ab; ORF1ab polyprotein) [Cleaved into: Host translation inhibitor nsp1; nsp1; Leader protein); Non-structural protein 2; nsp2; p65 homolog); Papain-like proteinase; PL-PRO; EC 3.4.19.12; EC 3.4.22.69; Non-structural protein 3; nsp3; PL2-PRO; SARS coronavirus main proteinase); Non-structural protein 4; nsp4); 3C-like proteinase; 3CL-PRO; 3CLp; EC 3.4.22.-; nsp5); Non-structural protein 6; nsp6); Non-structural protein 7; nsp7); Non-structural protein 8; nsp8); Non-structural protein 9; nsp9); Non-structural protein 10; nsp10; Growth factor-like peptide; GFL); RNA-directed RNA polymerase; Pol; RdRp; EC 2.7.7.48; nsp12); Helicase; Hel; EC 3.6.4.12; EC 3.6.4.13; nsp13); Guanine-N7 methyltransferase; ExoN; EC 2.1.1.-; EC 3.1.13.-; nsp14); Uridylate-specific endoribonuclease; EC 3.1.-.-; NendoU; nsp15); 2′-O-methyltransferase; EC 2.1.1.-; nsp16)]
• Protein Length: partial, Nsp13 function region
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

This multifunctional protein plays a critical role in the transcription and replication of viral RNAs. It contains proteinases that are responsible for cleaving the viral polyprotein into functional units. These cleavages are essential for the maturation of viral proteins and the progression of the viral life cycle. By facilitating the correct processing of the polyprotein, the protein ensures the production of the necessary components for viral replication. Its activity is pivotal in regulating the viral genome’s expression and maintaining the efficiency of the replication process, ultimately supporting the virus’s ability to infect and propagate within host cells.