Recombinant Human Sodium-dependent serotonin transporter (SLC6A4), partial

• Purity: >85% (SDS-PAGE)
• Host Species: Homo sapiens (Human)
• Accession: P31645
• Gene: SLC6A4
• CAT.NO: B000292
• Expression Systems: E.coli In Vivo Biotinylation in E.coli Yeast Baculovirus Mammalian cell
• Product Name: Recombinant Human Sodium-dependent serotonin transporter (SLC6A4), partial
• Source: E.coli;In Vivo Biotinylation in E.coli;Yeast;Baculovirus;Mammalian cell
• Tag: Tag type will be determined during the manufacturing process.
• Form: Lyophilized powder
• Buffer: Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Alternative Names: 5 HTT; 5 HTTLPR; 5 hydroxytryptamine (serotonin) transporter; 5 hydroxytryptamine transporter; 5HT transporter; 5HTT; hSERT; HTT; Na+/Cl- dependent serotonin transporter; OCD1; SC6A4_HUMAN; Serotonin transporter 1; SERT; SERT1; Slc6a4; Sodium dependent serotonin transporter; Sodium-dependent serotonin transporter; Solute carrier family 6 (neurotransmitter transporter) member 4; solute carrier family 6 (neurotransmitter transporter; serotonin); member 4; Solute carrier family 6 member 4
• Protein Length: Partial
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

The serotonin transporter is responsible for cotransporting serotonin along with one Na+ ion, one K+ ion, and possibly one proton in an electroneutral transport cycle. It moves serotonin across the plasma membrane from the extracellular space to the cytosol, thus limiting serotonin’s intracellular signaling. This transporter is crucial for maintaining serotonin homeostasis in the central nervous system. In the developing somatosensory cortex, it regulates serotonin uptake in glutamatergic neurons, influencing neuronal spatial organization and sensory circuit formation. In the mature cortex, it primarily functions in brainstem raphe neurons, terminating serotonin signaling by mediating its uptake from the synaptic cleft.