Recombinant Human Somatostatin Receptor Type 2 (SSTR2) Protein (His), Active

• Host Species: Human
• Accession: P30874
• Gene: SSTR2
• CAT.NO: B000042
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human Somatostatin Receptor Type 2 (SSTR2) Protein (His), Active
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Protein Expression Range: 1-369aa
• Tag: C-6His
• Molecular Mass: 42.5 kDa
• Form: Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
• Storage: 1. Store at -20～C/-80～C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4～C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20～C/-80～C. Protein in lyophilized powder form is stable for up to 12 months at -20～C/-80～C.
• Bioactivity: 1. Measured by its binding ability in a functional ELISA. Immobilized Human SSTR2 at 10 μg/mL can bind Anti-SSTR2 recombinant antibody , the EC 50 is 58.13-81.28 ng/mL. 2. Blocking experiment on Anti-SSTR2 antibody between Human SSTR2-VLPs protein and CT26/Human SSTR2 Stable Cells by Flow cytometry.

The somatostatin receptor binds somatostatin-14 and -28, coupling to pertussis toxin-sensitive G proteins to inhibit adenylyl cyclase. It also activates phosphotyrosine phosphatase and phospholipase C through both pertussis toxin-sensitive and -insensitive G proteins. The receptor suppresses calcium entry by inhibiting voltage-dependent calcium channels. It serves as the primary somatostatin receptor in pancreatic alpha- and beta-cells, mediating somatostatin-14’s inhibition of hormone secretion. Additionally, it regulates cell growth by enhancing MAPK1 and MAPK2 phosphorylation, leading to CDKN1B upregulation. In the brain, the receptor promotes neuronal migration and axon outgrowth, aiding in development and maturation. It also mediates negative regulation of insulin receptor signaling via PTPN6 and inactivates SSTR3 function through heterodimerization.