Recombinant Human T-Lymphocyte Activation Antigen Cd86 (CD86) Protein (His), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: P42081
• Gene: CD86
• CAT.NO: B000258
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human T-Lymphocyte Activation Antigen Cd86 (CD86) Protein (His), Active
• Endotoxin: Less than 1.0 EU/μg as determined by LAL method.
• Protein Expression Range: 24-247aa
• Tag: C-6His
• Molecular Mass: 26.69 kDa
• Form: Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.2
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.; 2. Avoid repeated freeze-thaw cycles.; 3. Store working aliquots at 4°C for up to one week.; 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: The ED50 as determined by its ability to bind Human CTLA-4 in functional ELISA is less than 20 ug/ml.
• Research Area: Immunology
• Protein Length: Extracellular Domain

This receptor plays a crucial role in providing the costimulatory signal necessary for T-lymphocyte proliferation and interleukin-2 production by binding to CD28 or CTLA-4. It is vital for the early stages of T-cell activation and costimulation of naïve T-cells, influencing the decision between immunity and anergy within the first 24 hours after activation. Additionally, it regulates B cell function and controls IgG1 production levels. Upon CD40 engagement, it activates the NF-kappa-B signaling pathway through phospholipase C and protein kinase C activation, contributing to immune response modulation and cellular signaling.