Recombinant Human Telomerase Reverse Transcriptase (TERT) Protein (His)

• Purity: Greater than 85% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: O14746
• Gene: TERT
• CAT.NO: B000207
• Expression Systems: E.coli
• Product Name: Recombinant Human Telomerase Reverse Transcriptase (TERT) Protein (His)
• Protein Expression Range: 281-436aa
• Tag: N-6His
• Molecular Mass: 22.7kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

Telomerase is a ribonucleoprotein enzyme crucial for replicating chromosome ends in most eukaryotes. It is active in progenitor and cancer cells but shows low or no activity in normal somatic cells. The enzyme’s catalytic component is part of the telomerase holoenzyme complex, which elongates telomeres by acting as a reverse transcriptase. It adds telomeric repeats (5′-TTAGGG-3′) to chromosome ends using an RNA template. Telomerase activity is regulated by associated proteins, chaperones, and modifiers. It is more efficient on substrates with 2-3 telomeric repeats and plays significant roles in aging, antiapoptosis, and modulating Wnt signaling.