Tubulin is the primary component of microtubules, cylindrical structures formed by laterally aligned protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubule growth occurs through the addition of GTP-bound tubulin dimers to the microtubule ends, creating a stabilizing GTP cap. This cap prevents depolymerization and stabilizes the growing microtubule. Below the cap, tubulin dimers exist in a GDP-bound state due to the intrinsic GTPase activity of beta-tubulin. The dynamic assembly and disassembly of microtubules play critical roles in cellular processes, including intracellular transport, cell division, and maintaining cellular shape.
| Product Name | Recombinant Human Tubulin Beta Chain (TUBB) Protein (His) |
| Accession | P07437 |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Host Species | Human |
| Gene | TUBB |
| Source | E.coli |
| Protein Expression Range | 1-444aa |
| Tag | C-6His |
| Molecular Mass | 50.6 kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
