Recombinant Human Tumor Necrosis Factor Receptor Superfamily Member 5 (CD40) Protein (hFc), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: P25942
• Gene: CD40
• CAT.NO: B000269
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human Tumor Necrosis Factor Receptor Superfamily Member 5 (CD40) Protein (hFc), Active
• Endotoxin: Less than 1.0 EU/μg as determined by LAL method.
• Protein Expression Range: 21-193aa
• Tag: C-hFc
• Molecular Mass: 46.3 kDa
• Form: Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.4
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.; 2. Avoid repeated freeze-thaw cycles.; 3. Store working aliquots at 4°C for up to one week.; 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: The ED50 as determined by its ability to bind Human TNFSF5 in functional ELISA is less than 20 ug/ml.
• Research Area: Immunology
• Protein Length: Extracellular Domain

This receptor binds TNFSF5/CD40LG and transduces signals mediated by TRAF6 and MAP3K8, which activate ERK in macrophages and B cells. This activation leads to the induction of immunoglobulin secretion, crucial for immune responses. By activating ERK, it plays a significant role in regulating immune cell functions, particularly in B cells, where it promotes the production of antibodies. The receptor’s involvement in these signaling pathways highlights its importance in immune activation and antibody production during immune responses, including inflammation and defense against pathogens.