ABL1 is a non-receptor tyrosine-protein kinase involved in critical cellular processes such as cytoskeleton remodeling, cell motility, adhesion, receptor endocytosis, autophagy, DNA damage response, and apoptosis. It regulates actin cytoskeleton dynamics by phosphorylating key proteins like WASF3 (branch formation), ANXA1 (membrane anchoring), DBN1, DBNL, CTTN, RAPH1, ENAH (signaling), MAPT, and PXN (microtubule-binding). Phosphorylation of WASF3 is essential for lamellipodia formation and cell migration.
ABL1 also modulates cell adhesion and motility by targeting regulators like BCAR1, CRK, CRKL, DOK1, EFS, and NEDD9. It phosphorylates multiple receptor tyrosine kinases, facilitating endocytosis of EGFR, neuromuscular synapse formation via MUSK, and inhibiting PDGFRB-mediated chemotaxis. Additionally, it regulates B-cell receptor complex endocytosis and phosphorylates caveolin (CAV1) and RIN1, which influence endocytic pathways.
ABL1 controls the activity of CBL ubiquitin ligases, promoting receptor down-regulation and actin remodeling. Phosphorylation of CBL enhances EGFR stability. It also supports late-stage autophagy by regulating lysosomal trafficking and function. During oxidative stress, ABL1 targets mitochondria, mediating dysfunction and cell death. It phosphorylates PRKD2 at Tyr-717 in response to oxidative stress, linking its activity to cellular survival and stress response pathways.
| Product Name | Recombinant Human Tyrosine-Protein Kinase Abl1 (ABL1) Protein (GST) |
| Accession | P00519 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Host Species | Human |
| Gene | ABL1 |
| Source | E.coli |
| Protein Expression Range | 4-194aa |
| Tag | N-GST |
| Molecular Mass | 48.1kDa |
| Form | Liquid or Lyophilized powder |
| Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Storage | 1. Store at -20¡«C/-80¡«C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4¡«C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20¡«C/-80¡«C. Protein in lyophilized powder form is stable for up to 12 months at -20¡«C/-80¡«C. |
