Recombinant Human Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1 (SIRPA) Protein (His), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: P78324
• Gene: SIRPA
• CAT.NO: B000251
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1 (SIRPA) Protein (His), Active
• Endotoxin: Less than 1.0 EU/μg as determined by LAL method.
• Protein Expression Range: 31-370aa
• Tag: C-6His
• Molecular Mass: 38.31 kDa
• Form: Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.2
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.; 2. Avoid repeated freeze-thaw cycles.; 3. Store working aliquots at 4°C for up to one week.; 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: The ED50 as determined by its ability to bind Human CD47 in functional ELISA is less than 0.5 ug/ml
• Research Area: Cardiovascular
• Protein Length: Partial

The immunoglobulin-like cell surface receptor for CD47 functions as a docking protein, facilitating the translocation of PTPN6, PTPN11, and other binding partners from the cytosol to the plasma membrane. It supports cerebellar neuron adhesion, neurite outgrowth, and glial cell attachment, playing a crucial role in synaptogenesis and synaptic function. Additionally, it negatively regulates receptor tyrosine kinase responses, phagocytosis, and immune cell activation. By binding CD47, it prevents dendritic cell maturation and cytokine production. This receptor is involved in antiviral immunity, limiting arenavirus infection by reducing virus internalization and mediating ROS signaling in response to THBS1.