Recombinant Human VEGFR1 Protein (C-Fc)

• Purity: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
• Host Species: Human
• Accession: P17948
• Gene: VEGFR1
• CAT.NO: B000058
• Expression Systems: Human Cells
• Product Name: Recombinant Human VEGFR1 Protein (C-Fc)
• Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
• Protein Expression Range: Ser27-Asn756
• Tag: C-Fc
• Molecular Mass: 109.3 KDa
• Buffer: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5%Trehalose, 5%Mannitol, 0.01%Tween80, pH7.0.
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Bioactivity: Immobilized Human VEGF165 at 10μg/ml (100 μl/well) can bind Human VEGFR1-Fc. The ED50 of Recombinant Human VEGFR1-Fc is 36.4 ng/ml. (Regularly tested)

This tyrosine-protein kinase functions as a cell-surface receptor for VEGFA, VEGFB, and PGF, playing a crucial role in embryonic vascular development, angiogenesis, cell survival, migration, macrophage function, chemotaxis, and cancer cell invasion. It positively regulates postnatal retinal hyaloid vessel regression and may act as a negative regulator of embryonic angiogenesis by controlling excessive endothelial cell proliferation.
In adulthood, it supports endothelial cell proliferation, survival, and angiogenesis in a cell-type-specific manner. It promotes PGF-induced endothelial cell proliferation and cancer cell growth but does not stimulate normal fibroblast proliferation (in vitro). With high VEGFA affinity and relatively low kinase activity, it may regulate VEGFA signaling by sequestering VEGFA and forming heterodimers with KDR to modulate KDR signaling.
Ligand binding activates multiple pathways, including PLCG, which generates diacylglycerol and inositol 1,4,5-trisphosphate, activating protein kinase C. It phosphorylates PIK3R1, triggering phosphatidylinositol kinase activity and downstream signaling. Additionally, it activates the MAPK/ERK and AKT1 pathways, phosphorylating AKT1 at ‘Ser-473’. It also mediates phosphorylation of SRC, YES1, CBL, and PTK2/FAK1. These diverse roles highlight its importance in vascular biology, cancer progression, and cellular signaling regulation.