Recombinant Influenza A virus Polymerase acidic protein (PA), partial

• Purity: >85% (SDS-PAGE)
• Host Species: Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
• Accession: P03433
• Gene: PA
• CAT.NO: B000249
• Expression Systems: E.coli In Vivo Biotinylation in E.coli Yeast Baculovirus Mammalian cell
• Product Name: Recombinant Influenza A virus Polymerase acidic protein (PA), partial
• Source: E.coli;In Vivo Biotinylation in E.coli;Yeast;Baculovirus;Mammalian cell
• Tag: Tag type will be determined during the manufacturing process.
• Form: Lyophilized powder
• Buffer: Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Alternative Names: PAPolymerase acidic protein; EC 3.1.-.-; RNA-directed RNA polymerase subunit P2
• Protein Length: Partial
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

This protein plays a crucial role in viral RNA transcription and replication by forming a heterotrimeric polymerase complex with PB1 and PB2 subunits. The complex transcribes viral mRNAs using a unique mechanism called cap-snatching, where it hijacks and cleaves host capped pre-mRNAs. These short, capped RNA fragments serve as primers for viral mRNA synthesis. The PB2 subunit binds to the 5′ cap of cellular pre-mRNAs, while the PA subunit, which carries endonuclease activity, cleaves the host RNAs after 10-13 nucleotides. This process is essential for the efficient replication of the viral genome within the host cell.