Recombinant Mouse 5'-Nucleotidase (NT5E) Protein (His), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Mus musculus (Mouse)
• Accession: Q61503
• Gene: NT5E
• CAT.NO: B000260
• Expression Systems: Mammalian cell
• Product Name: Recombinant Mouse 5'-Nucleotidase (NT5E) Protein (His), Active
• Endotoxin: Less than 1.0 EU/μg as determined by LAL method.
• Protein Expression Range: 29-550aa
• Tag: C-6His
• Molecular Mass: 58.8 kDa
• Form: Liquid
• Buffer: 0.2 μm filtered 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.; 2. Avoid repeated freeze-thaw cycles.; 3. Store working aliquots at 4°C for up to one week.; 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: Specific activity as determined by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP) is greater than 10000 pmol/min/ug. The orthophosphate product is measured by a malachite green phosphate detection kit.
• Research Area: Cardiovascular
• Protein Length: Partial

This enzyme catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside. It specifically hydrolyzes IMP and exhibits a preference for ribonucleotide monophosphates over their deoxyribonucleotide counterparts. While AMP is the preferred substrate, the enzyme is also capable of hydrolyzing other nucleotides such as UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD, and NMN. This versatility in substrate recognition highlights its broader role in nucleotide metabolism, contributing to the regulation of nucleotide levels and supporting various cellular processes involving nucleotides and nucleosides.