Recombinant Mouse Tumor Necrosis Factor Receptor Superfamily Member 14 (TNFRSF14) Protein (hFc), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Mus musculus (Mouse)
• Accession: Q80WM9
• Gene: TNFRSF14
• CAT.NO: B000272
• Expression Systems: Mammalian cell
• Product Name: Recombinant Mouse Tumor Necrosis Factor Receptor Superfamily Member 14 (TNFRSF14) Protein (hFc), Active
• Endotoxin: Less than 1.0 EU/μg as determined by LAL method.
• Protein Expression Range: 39-207aa
• Tag: C-hFc
• Molecular Mass: 45.6 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use.; 2. Avoid repeated freeze-thaw cycles.; 3. Store working aliquots at 4°C for up to one week.; 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: The ED50 as determined by its ability to bind Human BTLA in functional ELISA is typically 1.17 ug/ml
• Research Area: Cancer
• Protein Length: Partial

This receptor interacts with four distinct ligands: TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha, and immunoglobulin superfamily members BTLA and CD160, creating a complex network of stimulatory and inhibitory signaling. It signals through the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation. In antigen-presenting cells and lymphocytes, it participates in bidirectional cell-cell contact signaling. Upon TNFSF14/LIGHT ligation, it delivers costimulatory signals to T cells, enhancing proliferation and effector functions. It also interacts with CD160 on NK cells, boosting IFNG production and anti-tumor responses, and regulates immune responses on activated T cells.