Recombinant Saccharomyces cerevisiae 1,3-beta-glucan synthase component GSC2 (GSC2), partial

• Purity: >85% (SDS-PAGE)
• Host Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Accession: P40989
• Gene: GSC2
• CAT.NO: B000280
• Expression Systems: E.coli In Vivo Biotinylation in E.coli Yeast Baculovirus Mammalian cell
• Product Name: Recombinant Saccharomyces cerevisiae 1,3-beta-glucan synthase component GSC2 (GSC2), partial
• Source: E.coli;In Vivo Biotinylation in E.coli;Yeast;Baculovirus;Mammalian cell
• Tag: Tag type will be determined during the manufacturing process.
• Form: Lyophilized powder
• Buffer: Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Alternative Names: GSC2; FKS2; GLS2; YGR032W1,3-beta-glucan synthase component GSC2; EC 2.4.1.34; 1,3-beta-D-glucan-UDP glucosyltransferase; FK506 sensitivity protein 2; Glucan synthase of cerevisiae protein 2
• Protein Length: Partial
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

This alternate catalytic subunit of the 1,3-beta-glucan synthase (GS) complex is responsible for synthesizing 1,3-beta-glucan, a key structural component of the yeast cell wall. It is essential for the assembly of the spore wall. The enzyme’s activity is negatively regulated by SMK1, which is crucial for proper spore wall deposition. In contrast, its activity is positively regulated by RHO1, which enhances its function. Through these regulatory mechanisms, the catalytic subunit ensures the proper formation and structural integrity of both the yeast cell wall and the spore wall, supporting the yeast’s growth and reproduction.