Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep)

• Purity: Greater than 85% as determined by SDS-PAGE.
• Host Species: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
• Accession: P0DTD1
• Gene: rep
• CAT.NO: B000143
• Expression Systems: Baculovirus
• Product Name: Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep)
• Protein Expression Range: 1-601aa
• Tag: C-terminal 6xHis-tagged
• Molecular Mass: 70.0 kDa
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Research Area: Microbiology
• Alternative Names: (pp1ab)(ORF1ab polyprotein)(Leader protein)(Non-structural protein 1)(nsp1)(nsp2)(p65 homolog)(Non-structural protein 3)(nsp3)(PL2-PRO)(Papain-like proteinase)(PL-PRO)(nsp4)(3CL-PRO)(3CLp)(Main protease)(Mpro)(Non-structural protein 5)(nsp5)(SARS coronavirus main proteinase)(nsp6)(nsp7)(nsp8)(nsp9)(nsp10)(Growth factor-like peptide)(GFL)(Pol)(RdRp)(Non-structural protein 12)(nsp12)(Hel)(Non-structural protein 13)(nsp13)(ExoN)(Guanine-N7 methyltransferase)(Non-structural protein 14)(nsp14)(NendoU)(Non-structural protein 15)(nsp15)(Non-structural protein 16)(nsp16)
• Target Protein Sequence: AVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQ
• Protein Length: Full Length
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

nsp2 enhances the mRNA repression activity of the 4EHP-GYF2 complex in the host, thereby inhibiting the antiviral response and promoting SARS-CoV-2 replication. It likely works in cooperation with nsp1, which induces ribosome stalling on host mRNA, leading to a blockade in translation. This stalling triggers the host 4EHP-GYF2 complex to repress mRNA, a process that is further enhanced by nsp2. By interfering with host protein synthesis and immune response, nsp2 plays a crucial role in facilitating viral replication and helping the virus evade the host’s defense mechanisms.