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Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep) Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep)

Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep)

  • Purity: Greater than 85% as determined by SDS-PAGE.
  • Host Species: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
  • Accession: P0DTD1
  • Gene: rep
  • CAT.NO: B000143
  • Expression Systems:

    Baculovirus

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Product Name Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep)
Accession P0DTD1
Purity Greater than 85% as determined by SDS-PAGE.
Host Species Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Gene rep
Source Baculovirus
Protein Expression Range 1-601aa
Tag C-terminal 6xHis-tagged
Molecular Mass 70.0 kDa
Form Liquid or Lyophilized powder
Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Research Area Microbiology
Alternative Names (pp1ab)(ORF1ab polyprotein)(Leader protein)(Non-structural protein 1)(nsp1)(nsp2)(p65 homolog)(Non-structural protein 3)(nsp3)(PL2-PRO)(Papain-like proteinase)(PL-PRO)(nsp4)(3CL-PRO)(3CLp)(Main protease)(Mpro)(Non-structural protein 5)(nsp5)(SARS coronavirus main proteinase)(nsp6)(nsp7)(nsp8)(nsp9)(nsp10)(Growth factor-like peptide)(GFL)(Pol)(RdRp)(Non-structural protein 12)(nsp12)(Hel)(Non-structural protein 13)(nsp13)(ExoN)(Guanine-N7 methyltransferase)(Non-structural protein 14)(nsp14)(NendoU)(Non-structural protein 15)(nsp15)(Non-structural protein 16)(nsp16)
Target Protein Sequence AVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQ
Protein Length Full Length
Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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